CD44 is considered a cell-surface glycoprotein and is expressed on monocytes, lymphocytes, and granulocytes. It is also a homing cell adhesion molecule (H-CAM), and can also be an H-CAM for ECM-III, HUTCH-1, phagocytic glycoprotein-1, and Hermes-1. It can also be implicated in tumor metastasis, cell migration, and lymphocyte homing.
More Information
The CD44 antibody has a clone called SPM521, and the immunogen is the synthetic peptide that corresponds to the human protein of the same name. The isotype is the IgG20, and it has an undetermined epitope with a molecular weight of 80kDa.
Applications
This product can be used for Western Blotting and Immunohistochemistry applications.
For IHC applications, you’ll want to use a Formalin-fixed or paraffin-embedded tissue, as well as deparaffinized slides. You will find a concentrated formula that will require you to dilute it using a ratio of 1:50, though this is an estimate, as your protocols or methods may be different. However, if the ratio applies, you can also find a pre-diluted formula that is suitable for IHC applications.
To retrieve the antigen, you should boil the tissue sections using a Citrate buffer for 10 minutes and allow it to cool for 20 minutes to room temperature. Incubation periods are 30 minutes at room temperature, as well. The positive control is the esophageal carcinoma.
For Western Blotting, you should only use the concentrated formula. You’ll need to dilute the antibody using a ratio of 1:25, though this is an estimate. Incubation periods are one hour at room temperature. The positive control is the HeLa cell lysate.
Both applications have cellular localization occurring in the membrane.
The CD44 antibody is an excellent research tool to determine a variety of tumors and other situations in the human body. Visit Spring Bioscience now to learn more.
